An efficient method for the rapid establishment of Epstein‐Barr virus immortalization of human B lymphocytes
Open Access
- 28 August 2003
- journal article
- website
- Published by Wiley in Cell Proliferation
- Vol. 36 (4) , 191-197
- https://doi.org/10.1046/j.1365-2184.2003.00276.x
Abstract
Several methods have been developed for the immortalization of B lymphocytes by Epstein‐Barr virus (EBV). We developed an efficient method which reduces the time from culture initiation to immortalization and cryopreservation. Two infections of EBV to lymphocytes, and the use of phorbol ester‐induced EBV stock significantly improved immortalization efficiency and reduced the time between initiation and immortalization and cryopreservation. The resulting cell bank was used to produce DNA for genetic studies focusing on the genes involved in immune and autistic disorders.Keywords
This publication has 21 references indexed in Scilit:
- Protein Kinase C-Independent Activation of the Epstein-Barr Virus Lytic CycleJournal of Virology, 2002
- 12-O-Tetradecanoylphorbol-13-acetate Induces Epstein–Barr Virus Reactivation via NF-κB and AP-1 as Regulated by Protein Kinase C and Mitogen-Activated Protein KinaseVirology, 2001
- Soft Fibroma-Like Lesions on the Legs of a Patient with Kaposiʼs Sarcoma and LymphedemaThe American Journal of Dermatopathology, 1991
- Characteristics of Epstein-Barr virus activation of human B lymphocytes.The Journal of Experimental Medicine, 1981
- Long‐term T‐cell‐mediated immunity to Epstein‐Barr virus in man. III. Activation of cytotoxic T cells in virus‐infected leukocyte culturesInternational Journal of Cancer, 1979
- Long-term T-cell-mediated immunity to Epstein-Barr virus in man. II. Components necessary for regression in virus-infected leukocyte culturesInternational Journal of Cancer, 1979
- Long‐term T‐cell‐mediated immunity to epstein‐barr virus in man. I. Complete regression of virus‐induced transformation in cultures of seropositive donor leukocytesInternational Journal of Cancer, 1978
- Efficiency of transformation of lymphocytes by Epstein-Barr virusVirology, 1977
- Epstein-barr virus-induced transformation of human leukocytes after cell fractionationInternational Journal of Cancer, 1975
- Establishment of continuous lymphoblast cultures from leukocytes of gibbons(Hylobates lar)International Journal of Cancer, 1972