Isolation of a cDNA encoding the rat liver S‐adenosylmethionine synthetase
Open Access
- 30 September 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 184 (3) , 497-501
- https://doi.org/10.1111/j.1432-1033.1989.tb15042.x
Abstract
We have isolated cDNA clones encoding the rat liver S-adenosylmethionine synthetase by means of immunological screening from a phage λgt 11 expression library containing cDNA synthesized from adult rat liver poly(A)-RNA. The amino acid sequence deduced from the cDNA indicates that the rat liver enzyme for this protein contains 397 amino acid residues and has a molecular mass of 43697 Da. The deduced amino acid sequence of rat liver S-adenosylmethionine synthetase was 68% similar to those of yeast S-adenosylmethionine synthetases encoded by two unlinked genes SAM1 and SAM2. The rat liver S-adenosylmethionine synthetase also shows 52% similarity with the deduced amino acid sequence of the MetK gene encoding the S-adenosylmethionine synthetase in Escherichia coli.This publication has 26 references indexed in Scilit:
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