Respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay
- 1 September 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 12 (3) , 447-450
- https://doi.org/10.1128/jcm.12.3.447-450.1980
Abstract
A simplified enzyme-linked immunosorbent assay (ELISA) which utilized commercially available reagents was developed for respiratory syncytial virus (RSV)-specific IgG. An analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in RSV antibodies. The ELISA was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 RSV-infected adults. The ELISA correlated closely with complement fixation serological testing in 25 patients. The use of purified antigens might allow the development of a more sensitive ELISA.This publication has 3 references indexed in Scilit:
- Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infectionInfection and Immunity, 1978
- The enzyme-linked immunosorbent assay (ELISA): A measure of antibody concentration or affinity?Immunochemistry, 1978
- Quantitative, Semiautomated, Enzyme-Linked Immunosorbent Assay for Viral AntibodiesThe Journal of Infectious Diseases, 1977