A Simplified Stain for Hemoglobin in Tissues Or Smears Using Patent Blue
- 1 January 1946
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 21 (2) , 65-67
- https://doi.org/10.3109/10520294609110661
Abstract
Fix tissues in neutral formaldehyde soln., and embed in paraffin. Stain deparaffinized, hydrated sections 3 to 5 min. in the working reagent, prepared by adding 2 ml. glacial acetic acid and 1 ml. 3% H2O2 to 10 ml. filtered stock soln. (1 g. patent blue, 10 g. Zn powder, and 2 ml. glacial acetic acid). Counterstain 30-60 sec. in 1:1000 safranin soln. in 1 % acetic acid, rinse, dehydrate with alcohols, clear in xylene and mount in clarite. Total time required, 37 min. Useful for identifying Hb in tissues, also for blood smears after fixing in methyl alcohol.Keywords
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