Identification of Polypeptide Hormones in Tumors by Immunoelectron Microscopy

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Abstract
Immunoelectron microscopy is a very sensitive tool for locating small quantities of intracellular components. Selected polypeptide hormones were identified in tumor cells of humans by means of this technique. Immunoconjugated peroxidase, colloidal gold, and colloidal gold-conjugated protein A were employed as markers. Neutral-buffered formalin was determined to be an excellent fixative of hormone granules for immunolabeling, and for one case, hormone granules in tissues initially embedded in paraffin followed by reembedding in Spurr plastic were also shown to be immunoreactive.

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