The studies on the spherical bodies containing anthocyanins in plant cells. I. Cytological and cytochemical observations on the bodies appearing in the seedling hypocotyls of radish plants.

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Abstract
Microchemical and cytochemical tests were conducted on the red spherical bodies appearing in the seedling hypocotyls of a radish cultivar (Rhaphanus sativus L.), "Kohaku". In the fresh material of the hypocotyls the red body was broken down by the treatments with weak acids and alkalines, and also showed negative color change with Fehling''s reaction and iodine-potassium iodine reaction. In the paraffin sections clear stainings of the spherical bodies were obtained from the following dyes: safranin, both of basic and acidic fuchsins, fast green, methyl blue, orange G and aceto-carmine. However, negative results were given with methylene blue in both of alcohol and water, ruthenium red, sudan III, toluidine blue and nile blue. From these results it is conceivable that the spherical body has some special properties of cytoplasm. The relationship between the appearance of the body and the formation of anthocyanin was discussed.

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