Ouabain Stimulation of45Ca++Accumulation in the Rat Pituitary
- 1 May 1973
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 92 (5) , 1555-1559
- https://doi.org/10.1210/endo-92-5-1555
Abstract
Anterior pituitary tissue from rats was incubated at 37 C in Krebs' Ringer's bicarbonate in an atmosphere of 95% O2 and 5% CO2 with hourly changes of the incubation media. After a one hour preincubation, 45Ca++ was added to the media and its uptake by the pituitary tissue was determined. The digitalis glycoside, ouabain, in concentrations of 5 X 10-4 and 1 X 10-3M significantly augmented pituitary 45Ca++. This effect was dose and time dependent. Ouabain did not alter the washout kinetics for 14C inulin or affect pituitary weights suggesting that the observed changes in 45Ca++ content were the result of increased 45Ca++ accumulation. The effect of ouabain on calcium flux may be related to the stimulation of ACTH and GH release by ouabain. (Endocrinology92: 1555, 1973)Keywords
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