STUDIES ON A PHOSPHOLIPASE B FROM PENICILLIUM-NOTATUM - SUBSTRATE-SPECIFICITY

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 529  (1) , 29-37
Abstract
The action of a highly purified phospholipase B from P. notatum on 1-O-alk-1''-enyl-2-acyl-, 1-O-alkyl-2-acyl, 1,2-diacyl-, 1-acyl- and 2-acyl-sn-glycero-3-phosphocholine, monoacyl-, diacyl- and triacylglycerols, cholesteryl oleate and p-nitrophenyl acetate was studied. The hydrolysis products of the monoethermonoacylglycerophospholipids were identified as fatty acids, 1-O-alk-1''-enyl-sn-glycero-3-phosphocholine and 1-O-alkyl-sn-glycero-3-phosphocholine. The hydrolysis rates were in the following order: 1,2-diacyl-sn-glycero-3-phosphocholine > 1-O-alk-1''-enyl-2-acyl-sn-glycero-3-phosphocholine > 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine. 1-Acyl-sn-glycero-3-phosphocholine was hydrolyzed about 15 times faster than 2-acyl-sn-glycero-3-phosphocholine. Monoacylglycerols were hydrolyzed at the optima pH 4.0, but diacyl- and triacylglycerols were not hydrolyzed at various pH values between 4.0 and 9.0. Cholesteryl oleate and p-nitrophenyl acetate were not hydrolyzed.