Direct Demonstration of an Adaptive Constraint
- 20 October 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 314 (5798) , 458-461
- https://doi.org/10.1126/science.1133479
Abstract
The role of constraint in adaptive evolution is an open question. Directed evolution of an engineered β-isopropylmalate dehydrogenase (IMDH), with coenzyme specificity switched from nicotinamide adenine dinucleotide (NAD) to nicotinamide adenine dinucleotide phosphate (NADP), always produces mutants with lower affinities for NADP. This result is the correlated response to selection for relief from inhibition by NADPH (the reduced form of NADP) expected of an adaptive landscape subject to three enzymatic constraints: an upper limit to the rate of maximum turnover ( k cat ), a correlation in NADP and NADPH affinities, and a trade-off between NAD and NADP usage. Two additional constraints, high intracellular NADPH abundance and the cost of compensatory protein synthesis, have ensured the conserved use of NAD by IMDH throughout evolution. Our results show that selective mechanisms and evolutionary constraints are to be understood in terms of underlying adaptive landscapes.Keywords
This publication has 22 references indexed in Scilit:
- The Selective Cause of an Ancient AdaptationScience, 2005
- Natural Selection and Developmental Constraints in the Evolution of AllometriesScience, 2005
- Predicting Evolution by In Vitro Evolution Requires Determining Evolutionary PathwaysAntimicrobial Agents and Chemotherapy, 2002
- Developmental constraints versus flexibility in morphological evolutionNature, 2002
- Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilusJournal of Molecular Biology, 1997
- Ontoecogenophyloconstraints? The chaos of constraint terminologyTrends in Ecology & Evolution, 1991
- The spandrels of San Marco and the Panglossian paradigm: a critique of the adaptationist programmeProceedings of the Royal Society of London. B. Biological Sciences, 1979
- Selective disadvantage of non-functional protein synthesis inEscherichia coliJournal of Molecular Evolution, 1976
- Natural Selection and the Concept of a Protein SpaceNature, 1970
- Study of Microbial Evolution through Loss of Biosynthetic Functions: Establishment of “Defective” MutantsNature, 1967