Reversed Phase High-Performance Liquid Chromatography of Basic Drugs on a Silanol Deactivated Support

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Abstract
When polar and non-polar basic drugs were separated by reversed phase HPLC, a short-chain silanol deactivated (SCD-100) reversed phase column gave superior results over a standard C18 reversed phase column. The nature of silane, the type of silica and the chemistry of endcapping influenced chromatographic behavior. The peaks in the chromatogram obtained from this column had excellent peak shapes and eluted at predictable retention times, indicating that no silanols remained on the surface. The mobile phase was composed of phosphate buffer at pH 3.5, with variable amounts of methanol. All applications were isocratic without the addition of silanol suppressing reagents.