A Methylene Blue-Basic Fuchsin Stain for Mast Cells in Paraffin Sections
- 1 January 1962
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 37 (1) , 43-44
- https://doi.org/10.3109/10520296209114569
Abstract
In paraffin sections of rat tissue it is possible to stain mast cell granules blue in contrast to red nuclei, pale blue cytoplasmic ribonucleic acid, and colorless collagen. This is done by the following mixture: 1% methylene blue (pure, not polychrome), 9 ml; 0.1% basic fuchsin, 9 ml; glacial acetic acid, 2 ml. Stain formol-fixed, paraffin-processed sections for 5 min, wash in water and pass through acetone, 2 changes, 10 sec total, to xylene and a polystyrene mounting medium.Keywords
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