Bacterial Bioluminescence: Isolation and Expression of the Luciferase Genes from Vibrio harveyi
- 19 November 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 218 (4574) , 791-793
- https://doi.org/10.1126/science.10636771
Abstract
Genes for the luciferase enzyme of Vibrio harveyi were isolated in Escherichia coli by a general method in which nonluminous, transposon insertion mutants were used. Conditions necessary for light production in E. coli were examined. Stimulation of transcription of the genes for luciferase ( lux A and lux B ) was required for efficient synthesis of luciferase. To enhance transcription bacteriophage promoter elements were coupled to the cloned lux gene fragments.This publication has 11 references indexed in Scilit:
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