Identification of Xanthomonads and Grouping of Strains ofXanthomonas campestrispv.campestriswith Monoclonal Antibodies

Publisher Website
Google Scholar
Abstract
Monoclonal antibodies (MCA) were produced that identified the genus Xanthomonas, distinguished X. campestris pv. [pathovar] campestris (X. c pv. campestris) from other pathovars of X. campestris, and grouped and subgrouped strains of X. c. pv. campestris. One antibody, X1, was specific for all 436 xanthomonads that were tested, but it did not react with 104 bacteria of 12 other genera that were tested. Only crucifer strains (X. c. pv. campestris and 2 strains of X. c. pv. armoraciae) reacted with 1 or more of 3 MCA (X9, X13 and X17) whereas 192 xanthomonads of other species and pathovars failed to react with any of these MCA. The 104 strains of other genera and species also failed to react with these antibodies. Two hundred X. c. pv. campestris strains from Hawaii, Georgia, California, and Thailand were divided into 6 groups based on reactions with 5 MCA, and into subgroups based on these and other MCA. Most (83%) strains were in groups 1, 2, and 5; however, the strains from Hawaii and Georgia, the largest sample sizes, were distributed differently among these groups. These MCA may be applied as markers for rapid identification of X. c. pv. campestris strains and for tracing strains in epidemiological studies of black rot.