The effect of bisulfite modification on the template activity of DNA for DNA polymerase I1.
Open Access
- 1 July 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (7) , 889-900
- https://doi.org/10.1093/nar/1.7.889
Abstract
Cytosine residues of poly(C) and heat-denatured calf thymus DNA were transformed into 5, 6-dihydrouracil-6-sulfonate (U(SO −3 )) residues by treatment with bisulfite. The poly(U(SO −3 ) 2 , C 3 ) and poly(U(SO −3 ) 9 , C 1 ) prepared did not form inter-base binding with either poly(A) or poly(I) as judged by the absence of hypochromicity in ultraviolet absorbance. U(SO −3 ) residues in the DNA inactivated it to serve as template for E. coli DNA polymerase I, while the template activity was restored by conversion of the U(SO −3 ) residues into U.Keywords
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