The effects of tunicamycin on anterior pituitary hormone producing cells in the rat.
- 1 January 1984
- journal article
- research article
- Published by Japan Endocrine Society in Endocrinologia Japonica
- Vol. 31 (1) , 23-32
- https://doi.org/10.1507/endocrj1954.31.23
Abstract
An EM study was performed to clarify the effects of tunicamycin, a glycosylation inhibitor, on rat anterior pituitary cells. Tunicamycin (10, 50 and 100 .mu.g/250 g body wt) was i.p. injected into rats, which were sacrified 24 h later. Protein hormone-producing GH [growth hormone] and prolactin cells, and ACTH cells which are known to have a glycosylated precursor, showed no recognizable changes. TSH cells and gonadotrophs, both of which secrete glycoprotein hormones consisting of .alpha. and .beta. subunits, showed remarkable dilatation of the rough endoplasmic reticulum and decreased numbers of secretory granules. Apparently, the role of glycosylation in TSH cells and gonadotrophs may have a different biological significance from that in ACTH cells.This publication has 2 references indexed in Scilit:
- Immunocytochemical localization of glycoprotein hormones in the rat anterior pituitary. A light and electron microscope study using antisera against rat bet subunits: a comparison between preembedding and postembeeding methods.Journal of Histochemistry & Cytochemistry, 1980
- The Role of the Carbohydrate in the Stabilization, Processing, and Packaging of the Glycosylated Adrenocorticotropin-Endorphin Common Precursor in Toad PituitariesEndocrinology, 1979