Superparamagnetic Cation–Exchange Adsorbents for Bioproduct Recovery from Crude Process Liquors by High-Gradient Magnetic Fishing

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Abstract
Different routes were screened for the preparation of superparamagnetic cation–exchange adsorbents for the capture of proteins using high‐gradient magnetic fishing. Starting from a polyglutaraldehyde‐coated base particle, the most successful of these involved attachment of sulphite to oligomers of epichlorohydrin formed on the particle surface. The resultant cation‐exchanger had a maximum lysozyme binding capacity of 272 mg g−1 and a dissociation constant of 0.73 µM. Using lysozyme as a model protein in small‐scale studies, appropriate conditions were then selected for the capture of lactoperoxidase from sweet bovine whey. Subsequently, a high‐gradient magnetic fishing process was constructed for the fractionation of whey, in which lactoperoxidase was purified 36‐fold and concentrated 4.7‐fold.