The appearance and role of γδ T cells in the peritoneal cavity and liver during primary infection with Listeria monocytogenes in rats

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Abstract
We have previously reported that γδ T cells play important roles In protection during the early stage of infection with Listeria monocytogenes in mice. To generalize the protective roles of γδ T cells in listerial Infection to different species, we examined the appearance of γδ T cells during infection with L. monocytogenes In Fisher F344 rats. The numbers of bacteria In the peritoneal cavity and liver increased to a maximum level on day 3 and then decreased to an undetectable level by day 10 after an intraperitoneal infection with a subiethal dose (1 × 108) of viable L. monocytogenes in rats. CD3+αβ− T cells In the peritoneal cavity and liver began to Increase on day 3, reached a maximum level on day 6, and thereafter decreased gradually by day 10 after infection. Northern blot analysis confirmed that the CD3+αβ− T cells expressed TCR δ and γ gene messages. In vivo treatment with anti-TCRαβ mAb, which suppressed most of the αβ T cells in the periphery and impaired resistance during the late stage of listerial Infection, did not affect the host defense by day 6 after infection. A significantly Increased number of γδ T cells was detected in the peritoneal cavity of the TCRαβ-suppressed rats on day 6 after infection. These results suggest that the early appearing γδ T cells may contribute to the host defense at a relatively early stage during listeriosis in rats.

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