Effects of IL‐1 receptor‐associated kinase (IRAK) expression on IL‐1 signaling are independent of its kinase activity
Open Access
- 1 April 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 448 (1) , 81-85
- https://doi.org/10.1016/s0014-5793(99)00322-1
Abstract
Interleukin‐1 (IL‐1) stimulates the association of the IL‐1 receptor‐associated protein kinase (IRAK) with the heterodimer of IL‐1RI and IL‐1RAcP via the adapter protein MyD88. In the receptor complex IRAK becomes heavily phosphorylated and concomitantly activated. Here we show that overexpression of a kinase‐inactive mutant of IRAK (K239S) inhibits neither IL‐1‐stimulated activation of the transcription factor NF‐κB, nor that of the c‐Jun N‐terminal kinase nor IL‐2 production in murine EL‐4 cells, but enhances these effects in a manner comparable to wild type IRAK. This strongly suggests that the intrinsic kinase activity is not required for downstream signaling via IRAK.Keywords
This publication has 24 references indexed in Scilit:
- Activation of Nuclear Transcription Factor NF-κB by Interleukin-1 Is Accompanied by Casein Kinase II-mediated Phosphorylation of the p65 SubunitJournal of Biological Chemistry, 1997
- Phosphatidylinositol 3-Kinase in Interleukin 1 SignalingPublished by Elsevier ,1997
- Cytosolic domain of the type I interleukin-1 receptor spontaneously recruits signaling molecules to activate a proinflammatory gene.Journal of Clinical Investigation, 1997
- The Interleukin-1 Receptor Accessory Protein (IL-1RAcP) Is Essential for IL-1-induced Activation of Interleukin-1 Receptor-associated Kinase (IRAK) and Stress-activated Protein Kinases (SAP Kinases)Journal of Biological Chemistry, 1997
- A critical role for interleukin‐1 receptor accessory protein in interleukin‐1 signalingEuropean Journal of Immunology, 1997
- TRAF6 is a signal transducer for interleukin-1Nature, 1996
- NF-κB Activation by Interleukin-1 (IL-1) Requires an IL-1 Receptor-associated Protein Kinase ActivityPublished by Elsevier ,1995
- Interleukin‐1‐induced activation of a protein kinase co‐precipitating with the type I interleukin‐1 receptor in T cellsEuropean Journal of Immunology, 1994
- JNK1: A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domainPublished by Elsevier ,1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976