Production of recombinant hirudin in Hansenula polymorpha : variation of gene expression level depends on methanol oxidase and fermentation strategies
- 1 August 1998
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 21 (1-2) , 1-5
- https://doi.org/10.1038/sj.jim.2900545
Abstract
Various recombinant Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin. H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin. The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined.Keywords
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