Comparison of pulsed-field gel electrophoresis and ribotyping for subtyping ofVibrio choleraeO139 isolated in Thailand

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Abstract
Summary: Pulsed-field gel electrophoresis (PFGE) ofCpoI-digested genomic DNA and ribotyping (BglI) were applied to 60Vibrio choleraestrains including 48V. choleraeO139 from Thailand to compare their value in differentiating strains of the presentV. choleraeO139 epidemic. PFGE patters were divided into groups A and B representing five and four subtypes, respectively, while ribotyping showed four different patterns. PFGE group B subtypes were only presented among O139 isolates from Thailand, whereas four O139 strains from Bangladesh and India showed identical PFGE group A subtypes observed in O139 isolates from Thailand. Two nontoxigenic O139 isolates from Thailand showed different and unique PFGE types as did fiveV. choleraenon-Ol non-O139 isolates containing a gene virulence complex found inV. choleraeO139. These results indicate that PFGE (CpoI) can resolve recent evolutionary divergence withinV. choleraeO139 and offers a useful supplementary tool for following the progressingV. choleraeO139 epidemic.