Use of CpG oligodeoxynucleotides as immune adjuvants
Top Cited Papers
- 5 May 2004
- journal article
- review article
- Published by Wiley in Immunological Reviews
- Vol. 199 (1) , 201-216
- https://doi.org/10.1111/j.0105-2896.2004.00148.x
Abstract
Summary: Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs directly stimulate human B cells and plasmacytoid dendritic cells (pDCs), thereby promoting the production of T helper 1 (Th1) and pro‐inflammatory cytokines and the maturation/activation of professional antigen‐presenting cells. These activities enable CpG ODNs to act as immune adjuvants, accelerating and boosting antigen‐specific immune responses by 5–500‐fold. These effects are optimized by maintaining close physical contact between the CpG DNA and the immunogen. Animal challenge models establish that protective immunity can be accelerated and magnified by coadministering CpG DNA with vaccines. Ongoing clinical studies indicate that CpG ODNs are safe and well tolerated when administered as adjuvants to humans, and in some cases, they increase vaccine‐induced immune responses.Keywords
This publication has 132 references indexed in Scilit:
- Enhanced cell-mediated IFN-γ-secreting activity against the HIV-1IIIB V3 peptide of the TAB9 multiepitope after DNA vaccine backbone engineeringBiochemical and Biophysical Research Communications, 2003
- Local Delivery of CpG Oligodeoxynucleotides Induces Rapid Changes in the Genital Mucosa and Inhibits Replication, but Not Entry, of Herpes Simplex Virus Type 2Journal of Virology, 2003
- Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12European Journal of Immunology, 2001
- Subsets of Human Dendritic Cell Precursors Express Different Toll-like Receptors and Respond to Different Microbial AntigensThe Journal of Experimental Medicine, 2001
- Preserved epitope‐specific T cell activation by recombinant Bet v 1‐MBP fusion proteinsClinical and Experimental Allergy, 1998
- Macrophages sense pathogens via DNA motifs: induction of tumor necrosis factor‐α‐mediated shockEuropean Journal of Immunology, 1997
- CpG motifs in bacterial DNA trigger direct B-cell activationNature, 1995
- Induction of Immune-Mediated Glomerulonephritis in Normal Mice Immunized with Bacterial DNAClinical Immunology and Immunopathology, 1993
- Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral ProteinScience, 1993
- Theoretical and Experimental Approaches to Generalized AutoimmunityImmunological Reviews, 1990