Vibrational Imaging with High Sensitivity via Epidetected Coherent Anti-Stokes Raman Scattering Microscopy
- 20 June 2001
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 87 (2) , 023901
- https://doi.org/10.1103/physrevlett.87.023901
Abstract
We demonstrate theoretically and experimentally a novel epidetection scheme for coherent anti-Stokes Raman scattering (CARS) microscopy that significantly improves the detection sensitivity. Calculations show that epidetected CARS (E-CARS) signals are present for scatterers smaller than the wavelength of light, whereas the large background signals from the surrounding bulk solvent are suppressed by destructive interference. E-CARS microscopy is capable of revealing small intracellular features that are otherwise buried by the strong water CARS signal.Keywords
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