A modified technique for the determination of fungal mass in decayed wood

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Abstract
A modified technique to determine fungal mass within decayed wood is presented. The technique is an accurate, rapid method providing excellent separation of glucosamine from amino acids. Equal weights of pure mycelia of Coriolusversicolor (L. ex Fr.) Quél., Hirschioporusabietinus (Dicks, ex Fr.) Donk, and Poriaplacenta (Fr.) Cooke contained different amounts of glucosamine. Chipped forest residues, inoculated with the above basidiomycetes alone and in combination with yeasts, Pichiapinus (Holst) Phaff and Saccharomycesbailii Linder var. bailii, and nitrogen-fixing bacteria, Enterobacter spp., were assayed for glucosamine after 5 (laboratory) and 16 (field) months. More fungal mycelium was present when wood chips were inoculated with H. abietinus or P. placenta than in uninoculated chips. A twofold to threefold increase in glucosamine was observed when these basidiomycetes were combined with yeasts and bacteria.

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