Construction and Characterization of a Partial Library of Yeast Artificial Chromosomes from Human Chromosome 21
- 1 May 1991
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 10 (4) , 301-310
- https://doi.org/10.1089/dna.1991.10.301
Abstract
We report a protocol for cloning large DNA fragments in yeast artificial chromosomes (YAC). A partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human DNA. About 4.0 Mb of human DNA was recovered in 17 YAC clones. Three clones were analyzed by in situ hybridization and mapped on chromosome 21. One clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the localization of Alzheimer disease gene.Keywords
This publication has 49 references indexed in Scilit:
- Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.Proceedings of the National Academy of Sciences, 1990
- A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storageNucleic Acids Research, 1990
- DNA fingerprinting by sampled sequencing.Proceedings of the National Academy of Sciences, 1989
- Isolation of Single-Copy Human Genes from a Library of Yeast Artificial Chromosome ClonesScience, 1989
- Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresisNucleic Acids Research, 1989
- Characterization of the ‘unusual’ mobility of large circular DNAs in pulsed field-gradient electroplioresisNucleic Acids Research, 1988
- Transformation of yeast spheroplasts without cell fusionAnalytical Biochemistry, 1987
- Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome VectorsScience, 1987
- Defect in Alzheimer's is on Chromosome 21Science, 1987
- A simple and ranid method for preparing yeast chromosomes for Pulse Field Gel ElectrophoresisNucleic Acids Research, 1987