Spermatozoal Hapten Gained via Autolysis

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Abstract
Using autolysis different spermatozoa surface peptides were solubilized. The resulting low molecular weight compounds were further separated by gel-chromatography on Biogel P-4, thin-layer-chromatography on Cel 400 and thin-layer-electrophoresis. After the conjugation of the compound with cytochrome C a conformation-independent determinant was detected with immuno-electrophoresis. Out of the 17 fractions tested only fraction 3, with a molecular weight of 1800 daltons revealed a precipitation reaction with human sperm-immobilizing sera and rabbit antihuman sperm-serum. All 17 fractions showed no precipitation reaction against human sperm-agglutinating sera.