Abstract
1. Many drugs and foreign compounds contain nitrogen. Biological N-oxidation occurs with many types of N-containing compounds. 2. In mammals N-oxidation is catalyzed by enzyme systems in the endoplasmic reticulum of the liver and other tissues. Different mechanisms are involved in the N-hydroxylation of primary arylamines and the N-oxidation of secondary and tertiary amines. 3. Experimental data on the effects of stimulation and inhibition on microsomal N-oxidation of different types of amines are presented. 4. Kinetic parameters and pitfalls in experimental techniques of biological N-oxidation reactions are discussed.

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