Fractionation of Milk Proteins on Anion-Exchange Cellulose

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Abstract
An attempt was made to apply column chromatography, developed by Saber and Peterson, in fractionating milk proteins. The procedure used was that described by Saber et al. for preparing the adsorbent column and developing the chromatogram. Twenty-five milliliters of the dialyzed skimmilk was placed on the column (anion-exchange adsorbents DEAE-SF) and followed with continuous flow of buffer. Gradients of salt or pH or both were used for elution. Toluene-covered sodium phosphate buffers were used at flow rates of about 50 ml per hour (7 parts square inch N2). The individual effluent fractions of about 5 ml each were examined in a Beckman DU spectrophotometer at 280 mu. The elution diagram obtained is given.

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