Amplification of Coccidioidal DNA in Clinical Specimens by PCR

Open Access

1 May 2004

journal article
research article
Published by American Society for Microbiology in Journal of Clinical Microbiology

Vol. 42 (5) , 1982-1985
https://doi.org/10.1128/jcm.42.5.1982-1985.2004

Abstract

Coccidioides DNA was amplified from serum by a PCR using coccidioid-specific primers. A 239-bp product was visualized when 10 fg of exogenous coccidioidal DNA was subjected to amplification. This product was demonstrated in some human and mouse sera prior to the detection of coccidioidal antibodies.

Keywords

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