CELLULAR-HYPERSENSITIVITY TO NEOCARZINOSTATIN IN ATAXIA-TELANGIECTASIA SKIN FIBROBLASTS
- 1 January 1982
- journal article
- research article
- Vol. 42 (6) , 2247-2249
Abstract
Cellular sensitivity of human skin fibroblast strains from 3 healthy donors, 8 ataxia-telangiectasia (A-T) patients belongig to 6 sibships, and 2 A-T heterozygotes to the lethal action of the antitumor antibiotic neocarcinostatin was tested, using colony-forming ability as the criterion for survival. All the A-T strains were significantly more sensitive to killing by neocarzinostatin than were the control strains. The average D0 for the A-T strains following neocarzinostatin treatment was 14.6 ng/ml compared to 37.9 ng/ml for the normal strains. The 2 A-T heterozygous strains showed intermediate sensitivity with on average D0 of 26.9 ng/ml. Neocarzinostatin sensitivity of A-T cells could serve as a convenient aid for the laboratory diagnosis of A-T. Since A-T cells are also hypersensitive to ionizing radiation and bleomycin, it would appear that they are primarily hypersensitive to DNA-breaking agents.This publication has 4 references indexed in Scilit:
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