The activation of 50S and 30S E. coli ribosomes for polyphenylalanine synthesis.
- 1 January 1968
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 59 (1) , 238-245
- https://doi.org/10.1073/pnas.59.1.238
Abstract
The dissociated E. coli ribosomes which were relatively inactive in polyphenylalanine synthesis became very active after a temperature-dependent preincubation during which considerable association of the subunits occurred. Either ammonium of K ions were essential in the preincubation. When preincubation was done in the presence of spermidine and putrescine, the sub-units associated into particles 70S or larger which participated directly in synthesis without dissociating. Preincubation also enhanced the ability of the ribosomes to initiate polyphenylalanine synthesis with N-acetyl Phe-tRNA, only when unfractionated supernatant was included in the system.Keywords
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