An improved technique for the cryopreservation of Onchocerca microfilariae
- 1 August 1981
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 83 (1) , 139-146
- https://doi.org/10.1017/s0031182000050113
Abstract
SUMMARY: Experiments describe the use of ethanediol to store Onchocerca microfilariae in liquid nitrogen. The technique involves a 2-stage incubation of the parasites at 37 °C and 0 °C in ethanediol, before rapid cooling to − 196 °C. Viability has been assessed by motility, by migration in a proxy host and by development to the infective stage in the insect vector. A total of 71–79% of the cryopreserved microfilariae was shown to be viable compared to unfrozen controls. The technique is simple, inexpensive and very effective when compared to previous cryopreservation procedures for microfilariae and should be particularly suited for use under field conditions.This publication has 11 references indexed in Scilit:
- Schistosoma mansoni: Cryopreservation of schistosomula by two-step addition of ethanediol and rapid coolingExperimental Parasitology, 1981
- Laboratory meeting London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans 3rd May, 1979Transactions of the Royal Society of Tropical Medicine and Hygiene, 1980
- The Cryopreservation and in Vitro Cultivation of Larval Onchocerca VolvulusThe American Journal of Tropical Medicine and Hygiene, 1979
- Onchocerca spp: Cryopreservation of microfilariae and subsequent development in the insect hostExperimental Parasitology, 1979
- DemonstrationsTransactions of the Royal Society of Tropical Medicine and Hygiene, 1978
- Water transport and cell survival in cryobiological proceduresPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1977
- Cryoprotection—Permeant vs nonpermeant additivesCryobiology, 1974
- Studies onOnchocerca gutturosa(Neumann, 1910) and its Development inSimulium Ornatum(Meigen, 1818). 3. Factors Affecting the Development of the Parasite in its VectorJournal of Helminthology, 1973
- Changes in adaptation of a filarial worm, Brugia patei (Buckley, Nelson and Heisch), to a new mosquito host, Aedes togol (Theobald)Transactions of the Royal Society of Tropical Medicine and Hygiene, 1966
- The maintenance of Onchocerca gutturosa microfilariae in vitro and in vivoTransactions of the Royal Society of Tropical Medicine and Hygiene, 1966