INFLUENCES OF RECOVERY MEDIA AND INCUBATION TEMPERATURES ON THE TYPES OF MICROORGANISMS ISOLATED FROM SEAFOODS1

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Abstract
Four hundred fifty-five microbial isolates from seafoods were replicated on tryptone-peptone-yeast extract agar with 0.5% NaCl (TPE), trypticase soy agar with 3.0% NaCl (TSA), and plate count agar with no added NaCl (PCA). Daughter plates were incubated at 5, 25, 35, and 37 C, and colonies that developed on the plates were identified. At 25 C, 7% of TPE isolates failed to grow on PCA and 4% on TSA. The difference was mainly caused by Pseudomonas type III species, 24% of which failed to grow on PCA; Pseudomonas type II species, 13% of which failed on PCA; and Flavobacterium-Cutophaga species, 18% of which failed on TSA. Except for the reference mesophilic, cultures of Staphylococcus, Micrococcus, Escherichia coli, and Vibrio parahaemolyticus—which grew equally well at 25 and 35 C but failed to grow at 5 C—49% of colonies growing at 25 C failed to grow at 35 C, and 17% at 5 C. Microbial groups in order of sensitivity to 35 C were Arthrobacter, Pseudomonas, Moraxella, Micrococcus, Flavobacterium-Cytophaga, and Acinetobacter, with the respective growth failures at 35 C of 68, 51, 46, 42, 33, and 29%. Microbial groups that failed to grow at 5 C in the order of sensitivity were, Flavobacterium-Cutophaga, Acinetobacter, Arthrobacter, and Moraxella with respective growth failures of 40, 26, 12, and 9%.

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