High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA
- 1 August 1986
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 52 (2) , 394-395
- https://doi.org/10.1128/aem.52.2.394-395.1986
Abstract
Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).This publication has 10 references indexed in Scilit:
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