APPLICATION OF A SPECTROPHOTOMETRIC METHOD TO THE DETERMINATION OF POTASSIUM PENICILLIN, PROCAINE PENICILLIN AND BENZATHINE PENICILLIN IN PHARMACEUTICAL PREPARATIONS

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Abstract
A spectrophotometric method originally designed for the estimation of potassium penicillin has been applied to procaine penicillin and benzathine penicillin. A solution containing the penicillin salt is heated under controlled conditions with a sodium acetate-acetic acid buffer solution of pH 4ṁ6 to which a trace of copper sulphate has been added. The optical absorption of the resulting penicillinic acid is measured on a spectrophotometer. The method has a standard error of 3 per cent and is applicable to a variety of penicillin preparations ranging in potency from 0ṁ5 units/mg. upwards.