Calmyonemin: Identification and distribution throughout thecell cycle in Entodinium bursa (ciliate)

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Abstract
Summary— The ciliature in most entodiniomorphid ciliates is restricted to the cell apex, forming ciliary crowns that can be rapidly retracted under stress conditions. In a previous study, calmyonemin, a 23 kDa analog of centrin, has been characterized from ciliated cortical zones in Eudiplodinium maggii; its localization in subkinetal myonemes, ie bundles of contractile filaments powering retraction of the ciliature, was demonstrated by immunoelectron microscopy (David and Vigues (1994) Cell Motil Cytoskeleton, 27, 169–179). Here we used immunofluorescence microscopy to determine the distribution of calmyonemin and its variations throughout the cell cycle in Entodinium bursa. Labelling of morphostatic cells with anti‐calmyonemin antibody reveals immunoreactivity at the base of the ciliature, in circumciliary lips, around the cytoproct and in an endoplasmic array originating from the post‐oral region and extending down to the posterior part of the cell. All arrays of calmyonemin from the parental cell remain unaffected during cell division except the endoplasmic array which undergoes a transient resorption followed by synchronous reassembly in the two future products of division. The assembly of subkinetal myonemes in the sub‐equatorial zone is described in relation with the main features of oral morphogenesis previously revealed by others using silver impregnation techniques and light microscopy.

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