Relationship between Blood Clearance and Viruria after Intravenous Injection of Mice and Rats with Bacteriophage and Polioviruses

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Abstract
Summary: The relationship between viremia and viruria occurring within 2 hr after intravenous injection with T2 bacteriophage and polioviruses was studied in mice and rats. Poliovirus was cleared from the blood at a slower rate than phage. Blood specimens obtained at 2 hr contained approximately 104 less phage particles but only 101.8 to 102.6 TCID50 less poliovirus than inoculated. Viruria could not be demonstrated if less than 109 phage particles or 105 TCID50 of poliovirus were injected. With larger doses of virus, the incidence of viruria increased and the amount of virus in the urine tended to increase. A gradient of at least 105:1 was observed between blood and urine concentrations of poliovirus and phage. This type of viruria occurring within 2 hr after intravenous injection of large doses of virus was designated “immediate viruria.” Despite bilateral ureteral interruption in mice and rats, poliovirus viruria was demonstrated in amounts comparable to those found in sham-operated or intact animals.