DC Polarography of Cephradine and Its Application to Capsules
- 1 May 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 62 (3) , 556-559
- https://doi.org/10.1093/jaoac/62.3.556
Abstract
A polarographic method has been developed for the quantitative analysis of cephradine and its dosage forms. Direct determinations on capsules are carried out; excipients and coloring matter do not interfere in the determination. The electroactive product is formed by acidic hydrolysis with 5.0N HCl and heating at 80°C for 60 min. Two polarographic waves are obtained: I = - 0.46 v and II = - 0.78 v vs. SCE. Both reduction waves are diffusion controlled. Wave I is preferred for analytical purposes. The precise chemical identity of the electroactive product has not been determined, but UV spectral data and the TLC Rf value are reported. A linear relation is established for levels of cephradine between 10-2 and 10-5M in 5.0N HCl.This publication has 0 references indexed in Scilit: