Separation of Serum Antibody Activities by Anion-Exchange Cellulose Chromatography

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Abstract
Mumps, Histoplasma capsulatum. thyroglobulin, and typhoid H antibodies were found in fraction 1, which contained only gamma globulins with ultracentrifugal sedimentation coefficients of 6.6 Svedberg units (S). Typhoid O antibodies and rheumatoid factor were only in fraction 5, composed principally of gamma macroglobulins with ultracentrifugal sedimentation coefficients of 18 S. In contrast isohemagglutinins, Rh antibodies, and anti-human liver nucleoprotein activities were found in two chromatogram fractions (1 and 5) with different physicochemical properties.