A model for the computer analysis of synchronous DNA distributions obtained by flow cytometry
- 1 July 1980
- Vol. 1 (1) , 71-77
- https://doi.org/10.1002/cyto.990010114
Abstract
A new model for obtaining the distributions of cells in the cell cycle by computer analysis of flow cytometry data is proposed. The model is able to analyze distributions from both asynchronous and synchronous cell populations. A culture of HeLa cells is pulse‐labeled with tritiated thymidine at various times after sychronization by the mitotic selection method, then analyzed by flow cytometry and autoradiography. The results show good agreement between the percentage S‐phase cells determined by the model and by autoradiography.Keywords
This publication has 19 references indexed in Scilit:
- Enhanced light collection in a flow cytometerCytometry, 1980
- Selection of optimal model for the DNA histogram by analysis of error of estimated parameters.Journal of Histochemistry & Cytochemistry, 1979
- Quantitative Single Cell Analysis and SortingScience, 1977
- Mathematical methods for determining cell DNA synthesis rate and age distribution utilizing flow microfluorometryJournal of Theoretical Biology, 1977
- Chromomycin A3 as a fluorescent probe for flow cytometry of human gynecologic samples.Journal of Histochemistry & Cytochemistry, 1977
- Analysis of deoxyribonucleic acid histograms from flow cytofluorometry. Estimation of the distribution of cells within S phase.Journal of Histochemistry & Cytochemistry, 1977
- Quantitative analysis of flow microfluorometric data from asynchronous and drug-treated cell populationsComputers and Biomedical Research, 1976
- Method for the quantitative evaluation of data from flow microfluorometryComputers and Biomedical Research, 1976
- CELL CYCLE ANALYSIS FROM COMPUTER SYNTHESIS OF DEOXYRIBONUCLEIC ACID HISTOGRAMSJournal of Histochemistry & Cytochemistry, 1974
- Cell Microfluorometry: A Method for Rapid Fluorescence MeasurementScience, 1969