A Screening Assay for the Tetrachlorodibenzo-p-dioxin Receptor using the [125I]iodovaleramide Derivative of Trichlorodibenzo-p-dioxin as the Binding Ligand
- 1 June 1984
- journal article
- research article
- Published by Taylor & Francis in International Journal of Environmental Analytical Chemistry
- Vol. 17 (3-4) , 267-274
- https://doi.org/10.1080/03067318408076978
Abstract
A relatively simple assay method for the putative cytosolic ‘receptor’ that binds 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and related compounds is described. The assay is based on specific binding of [125I]dioxin to cytosol ‘receptor’ protein. Saturation is ensured by competition experiments in which unlabeled TCDD and other competitors displace the radiolabeled ligand from specific binding sites. This assay has been applied to estimation of levels of ‘receptor’ in cytosol.Keywords
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