Requirement for a reduced sulphydryl entity in the protection of molybdate‐stabilized estrogen receptor
- 1 November 1982
- journal article
- Published by Wiley in FEBS Letters
- Vol. 149 (1) , 80-84
- https://doi.org/10.1016/0014-5793(82)81076-4
Abstract
The separate and combined effects of molybdate and dithiothreitol on the stability of human uterine 9 S estrogen receptor were studied. Maximal, short-term, protection of the 9 S estrogen receptor was achieved by the joint inclusion of both stabilizing agents in cytosol buffers. This molybdate—dithiothreitol-mediated stability was dependent on reducing agent concentration inferring sulphydryl involvement in 9 S receptor protection by molybdate. The study also showed that molybdate—dithiothreitol could not prevent the gradual decay of the 9 S estrogen receptor to the 4 S form in cytosols stored at 4°C over prolonged periods.Keywords
This publication has 16 references indexed in Scilit:
- The influence of leupeptin, molybdate and calcium ions on estrogen receptor stabilityFEBS Letters, 1981
- Role of sulfhydryl modifying reagents in the binding and activation of chick oviduct progesterone-receptor complexThe Journal of Steroid Biochemistry and Molecular Biology, 1981
- Protection of steroid hormone receptors by protease inhibitorsBiochemical and Biophysical Research Communications, 1981
- Estrogen Receptor Cleavage and Plasminogen Activation by Enzymes in Human Breast Tumor Cytosol*Endocrinology, 1980
- Inhibition of progesterone receptor activation by sodium molybdateBiochemistry, 1980
- The chemical evolution of a nitrogenase model. 17. Simulation of steric and of inhibitory effects at the enzymic active site with acetylenes and nitriles as the substrates, and "molybdoinsulin" catalystsJournal of the American Chemical Society, 1979
- The involvement of receptor sulphydryl groups in the binding of steroids to the cytoplasmic glucocorticoid receptor from rat thymusBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- The protection, stabilization and reactivation of estradiol receptors in human myometrial cytosolBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Estrogen receptors in human breast cancer: 1. Methodology and characterization of receptorsSteroids, 1971
- Sulfhydryl Groups and Estradiol-Receptor InteractionScience, 1967