USE OF 8-AZAGUANINE FOR THE ISOLATION OF AUXOTROPHIC MUTANTS OF BACILLUS MEGATERIUM
- 1 January 1962
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 83 (1) , 35-+
- https://doi.org/10.1128/jb.83.1.35-37.1962
Abstract
8-Azaguanine is bactericidal for Bacillus megaterium strain KM, primarily under conditions where growth is possible. This analogue has been used to enrich for auxotrophic mutants in ultraviolet-irradiated populations. The survivors were found to contain from 1.6 to 33.0% amino acid auxotrophs and from 19 to 29% pyrimidine nucleoside auxotrophs. Since many different biological systems are sensitive to 8-azaguanine, the use of this analogue to enrich for auxotrophic mutants may have a more general application than techniques currently employed.Keywords
This publication has 12 references indexed in Scilit:
- EFFECT OF 8-AZAGUANINE ON GROWTH AND VIABILITY OF BACILLUS MEGATERIUMJournal of Bacteriology, 1962
- Selecting Bacterial Mutants by the Penicillin MethodScience, 1960
- EFFECTS OF HEATING DRY BACTERIA AND SPORES ON THEIR PHENOTYPE AND GENOTYPEProceedings of the National Academy of Sciences, 1960
- The specific mutagenic effect of base analogues on Phage T4Journal of Molecular Biology, 1959
- Selection of Auxotrophic Bacterial Mutants by Tritium-Labeled ThymidineScience, 1959
- Selection of Auxotrophic Bacterial Mutants through Diaminopimelic Acid or Thymine DeprivalScience, 1957
- The metabolism of 8-azapurinesBiochemical Journal, 1957
- CONCENTRATION OF BIOCHEMICAL MUTANTS OF BACTERIA WITH PENICILLIN1Journal of the American Chemical Society, 1948
- ISOLATION OF BIOCHEMICALLY DEFICIENT MUTANTS OF BACTERIA BY PENICILLINJournal of the American Chemical Society, 1948
- Experiments with Different Methods of Isolating Physiological Mutations of Filamentous FungiNature, 1947