Morphological and functional in vitro and in vivo characterization of the mouse corpus cavernosum
- 1 March 2001
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 132 (6) , 1333-1341
- https://doi.org/10.1038/sj.bjp.0703938
Abstract
1. In normal mice, the distribution of adrenergic, cholinergic, some peptidergic, and neuronal nitric oxide synthase (nNOS)-containing nerves were investigated. Functional in vitro correlates were obtained. An in vivo model was developed in which erectile haemodynamics in response to drugs or nerve-stimulation were studied. 2. Immunoreactivities for vesicular acetylcholine transporter protein (VAChT), nNOS-, and vasoactive intestinal polypeptide (VIP), co-existed in nerve fibres and terminal varicosities. Immunoreactivities for neuropeptide Y (NPY) and tyrosine hydroxylase (TH) were found in the same nerve structures. 3. Chemical sympathectomy abolished TH- and NPY-IR nerve structures in cavernous smooth muscle bundles. The distribution of calcitonin gene-related peptide (CGRP)-, nNOS-, VAChT- and VIP-IR nerve structures was unchanged. 4. In endothelial cells of the central and helicine arteries, veins and venules, intense immunoreactivity for endothelial NOS (eNOS) was observed. No distinct eNOS-IR cells were found lining the cavernous sinusoids. 5. In vitro, nerve-induced relaxations were verified, and endothelial NO/cyclic GMP-mediated relaxant responses were established. VIP and CGRP had small relaxant effects. A functioning adenylate cyclase/cyclic AMP pathway was confirmed. 6. Neuronal excitatory responses were abolished by prazosin, or forskolin. VIP and CGRP counteracted contractions, whereas NPY and scopolamine enhanced excitatory responses. 7. In vivo, erectile responses were significantly attenuated by L-NAME (50 mg kg(-1)) and facilitated by sildenafil (200 microg kg(-1)). 8. It is concluded that the mouse is a suitable model for studies of erectile mechanisms in vitro and in vivo.Keywords
This publication has 33 references indexed in Scilit:
- CHOLINERGIC NERVES IN HUMAN CORPUS CAVERNOSUM AND SPONGIOSUM CONTAIN NITRIC OXIDE SYNTHASE AND HEME OXYGENASEJournal of Urology, 2000
- THE EFFECT OF SILDENAFIL ON APOMORPHINE-EVOKED INCREASES IN INTRACAVERNOUS PRESSURE IN THE AWAKE RATJournal of Urology, 1999
- NO synthase in cholinergic nerves and NO‐induced relaxation in the rat isolated corpus cavernosumBritish Journal of Pharmacology, 1999
- EFFECT OF THE SELECTIVE PHOSPHODIESTERASE TYPE 5 INHIBITOR SILDENAFIL ON ERECTILE FUNCTION IN THE ANESTHETIZED DOGJournal of Urology, 1998
- Distribution and function of nitric oxide‐containing nerves in canine corpus cavernosum and spongiosumActa Physiologica Scandinavica, 1995
- Effects of the nitric oxide synthase inhibitor NG‐nitro‐L‐arginine on the erectile response to cavernous nerve stimulation in the rabbitActa Physiologica Scandinavica, 1991
- Neurophysiological Aspects of Penile Erection: the Role of the Sympathetic Nervous SystemBritish Journal of Urology, 1989
- Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factorNature, 1987
- PRE‐ AND POSTJUNCTIONAL ADRENO‐ AND MUSCARINIC RECEPTOR FUNCTIONS IN THE ISOLATED HUMAN CORPUS SPONGIOSUM URETHRAEJournal of Autonomic Pharmacology, 1984
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980