Change in the Ultraviolet Spectrum of Solubilized Ca2+-Dependent ATPase from Sarcoplasmic Reticulum due to Binding with Ca2+Ions1
- 1 August 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 86 (2) , 443-446
- https://doi.org/10.1093/oxfordjournals.jbchem.a132542
Abstract
Solubilized sarcoplasmic reticulum (SSR) was prepared by solubilizing fragmented sarcoplasmic reticulum (FSR) with a nonionic detergent (C12E8) then displacing the detergent with Tween 80, using a DEAE-cellulose column. The UV absorption of SSR decreased reversibly at about 286 and 292 nm on removal of free Ca2+ions, while no change in the fluorescence spectrum was detectable. On the other hand, the fluorescence intensity of FSR decreased 3–4% on removal of free Ca2+ions, as previously reported by Dupont [(1976) Biochem. Biophys. Res. Commun. 71, 544–550]. The UV absorption of FSR increased reversibly at about 270–280 nm on removal of free Ca2+ions, but the rate of the change was very slow (k=about 0.1 min−1).Keywords
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