Rapid detection ofVibrio cholerae O∶1 by motility inhibition and immunofluorescence with monoclonal antibodies

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Abstract
Monoclonal antibodies against the group and type specific antigens ofVibrio cholerae O∶1 lipopolysaccharide were used for the rapid detection ofVibrio cholerae strains by motility inhibition and immunofluorescence. Motility inhibition of liveVibrio cholerae O:1 was obtained with group specific monoclonal antibodies. Monoclonal antibodies against the type specific antigens B (Ogawa) and C (Inaba) inhibited motility of strains of homologous serotypes only. Indirect immunofluorescence of heatfixed bacteria with monoclonal antibodies and fluorescein-isothiocyanate conjugated rabbit anti-mouse immunoglobulin was also shown to be suitable for the rapid detection ofVibrio cholerae O∶1. Both tests were highly specific and no cross-reactions were observed with strains of non-O∶1 vibrios,Escherichia coli orSalmonella spp. tested. However, a weak fluorescence of some Ogawa strains was observed when high concentrations of Inaba specific monoclonal antibodies were used.