Development of genetic markers in celery based on restriction fragment length polymorphisms
- 1 February 1993
- journal article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 85-85 (6-7) , 889-896
- https://doi.org/10.1007/bf00225034
Abstract
Linkage relationships are reported for 34 markers in celery (Apium graveolens L. var ‘dulce’) including 21 RFLP, 11 isozyme, and 2 morphological traits. The mapping was carried out in a cross between celery and an annual accession from Thailand, A143, and based on F2 segregation of 136 plants. A total of 318 centiMorgans (cM) are covered by the markers distributed in 8 linkage groups. Probes for the identification of RFLPs were isolated from a celery cDNA library and were also obtained from heterologous sources. EcoRV, EcoRI, and HindIII were the most useful restriction enzymes in uncovering polymorphism. In our cross, 18% of the cDNA probes were found to be polymorphic for at least one of the enzymes used. Six of the markers showed significant deviations from expected F2 ratios.Keywords
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