Isolation and in vitro Translation of mRNA from Inflorescences of Parietaria judaica

Abstract
The aqueous extract of inflorescences of Parietaria judaica contains an allergen homologous to the major pollen allergen Par·I (14 kD), as shown by radio-allergosorbent test (RAST) inhibition and immunoblot analysis. Poly(A)+ RNA was obtained from inflorescences and was shown to be able to code in vitro for a protein homologous to Par·I with respect to sodium dodecylsulphate polyacrylamide gel electrophoretic mobility and to antigenic specificity as defined by the binding, in affinity chromatography, to solid-phase IgG of rabbit anti- Par·I antisera, and in RAST inhibition, to IgE antibodies of human reaginic serum pool.