Identification of Crustacea Allergens by Crossed Radioimmunoelectrophoresis

Publisher Website
Google Scholar
Abstract
Crossed immunoelectrophoresis (CIE) detected 18 precipitating antigens in extracts of shrimp. Of these antigens, crossed-line immunoelectrophoresis (CLIE) of shrimp extract demonstrated that 5 cross-reacted with crawfish, 3 with lobster and 1 with crab extract. Allergens present in the shrimp CIE plates were identified by crossed radioimmunoelectrophoresis (CRIE) using sera from 6 study subjects who were skin-test and RAST positive to shrimp extract. Of the 7 allergens detected, 3 (precipitins 1, 3 and 6) reacted with most of the 6 sera tested from shrimp-sensitive subjects. Precipitins 1 and 6 appear to be common Crustacea allergens (present in shrimp, crawfish, lobster and crab) whereas precipitin 3 may be a specific allergen since it is present only in shrimp.

This publication has 0 references indexed in Scilit: