Antibody response to embryonal carcinoma cells in syngeneic mice

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Abstract
The immunoglobulin (Ig) classes and subclasses of the specific antibodies contained in antisera raised in male 129/Sv mice against cells of a syngeneic clonal line of embryonal carcinoma (F9) have been determined. Cytotoxic activity was found associated almost exclusively with anti‐F9 IgM (μχ) antibodies. A large part of anti‐F9 activity was found associated with IgG1 (γ1χ and presumably also γ1λ) antibodies, and was detectable only by indirect immunofluorescence. Traces of specific IgG2a and IgG2b antibodies were also found. No IgG3 and IgA antibodies reacting with embryonal carcinoma cells were detectable under these conditions. The serum of F9 tumor‐bearing animals had a very similar Ig composition. fürthermore, IgM and IgG1, but not IgG2 antibodies, were detected at the surface of in vivo growing F9 tumor cells.