SEQUENTIAL SYNTHESES OF VARIOUS MOLECULAR SPECIES OF RIBONUCLEIC ACID IN THE EARLY PHASE OF CONIDIA GERMINATION IN ASPERGILLUS ORYZAE

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Abstract
RNA synthesis in early stages of germination of Aspergillus oryzae conidia was studied using ortho-phosphate-p32 or C14O2 as precursor. RNA was fractionated by the methylated albumin-coated kieselguhr column chromatography or by the sucrose density gradient centrifugation analysis. RNA synthesis occurred at any time of germination so far observed and tRNA or its precursor was synthesized at the initial step of germination. sRNA synthesis occurred a little later than the onset of germination and mRNA synthesis became apparent after 60 min. of incubation. Even in non-germinating medium (phosphate buffer), conidia could synthesize rRNA. It was suggested that the synthesis of rRNA or its precursor may be one of the starting reactions being driven at the very initial step of germination.